Journal: Pharmaceutics

Article Title: Minoxidil Nanoparticles Targeting Hair Follicles Enhance Hair Growth in C57BL/6 Mice

doi: 10.3390/pharmaceutics14050947

Figure Lengend Snippet: Microscopic effects of MXD formulations on skin tissue (hair bulge and hair bulb) of C57BL/6 mice at 8 d after the start of repetitive applications (once a day). ( A ) Images of H&E-stained skin tissue specimens from C57BL/6 mice (bars indicate 100 µm). ( B ) High magnification microscope images in the areas designated by the squares in Figure A (bars indicate 50 µm). ( C ) Optical images of immunostaining for CD200 in the serial segments shown in Figure A (bars indicate 100 µm). ( D ) High magnification microscope images of the areas designated by the squares in Figure C (bars indicate 50 µm). Vehicle, only the vehicle used to prepare the MXD-MPs and MXD-NPs formulations was applied. MXD-MPs, MXD-MPs-applied mice. MXD-NPs, MXD-NPs-applied mice. CA-MXD, CA-MXD-applied mice. The arrow shows the expression of CD200. CD200 is stained brown; the expression of CD200 was enhanced in the MXD-NPs group, compared with the CA-MXD group.

Article Snippet: Paraffin sections were obtained from the fixed mouse back skin tissues in the usual manner and the product certificate and were incubated with anti-CD200 rabbit polyclonal antibody (1:100; Cat No. 14057-1-AP; Proteintech, Rosemont, IL, USA) for 1 h at 37 °C.

Techniques: Staining, Microscopy, Immunostaining, Expressing

Journal: Cell Reports Medicine

Article Title: In vivo vulnerabilities to GPX4 and HDAC inhibitors in drug-persistent versus drug-resistant BRAF V600E lung adenocarcinoma

doi: 10.1016/j.xcrm.2024.101663

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-CD200 (clone E519V) , Cell signaling Technology , Cat#23451; RRID: AB_3102027.

Techniques: Recombinant, Glutathione Assay, Flow Cytometry, shRNA, Control, Plasmid Preparation, Software

Journal: International journal of cosmetic science

Article Title: Mechanical epilation exerts complex biological effects on human hair follicles and perifollicular skin: An ex vivo study approach.

doi: 10.1111/ics.12923

Figure Lengend Snippet: FIGURE 8 Hair follicle (HF) immune privilege is transiently perturbed by epilation. (a, b) Quantitative immuno-histomorphometry of MHC class I, depicted as fold change of relative staining intensity, normalized to the respective control group of each day, in the hair bulb (a) and bulge (b) and CD200 expression, depicted as fold of relative staining intensity, normalized to the respective control group of each day in the bulge (c) at days 0, 3, and 6 post-epilation. The relative staining intensity of each protein was measured in the specific reference area of the sections. N = 2–15 HFs/group from one independent experiment (one donor, two punches per experimental group). Mean ± SEM. Data were analysed with a D'Agostino & Pearson normality test. If data were normally distributed, an unpaired Student's t-test was used (CD200); if data were not normally distributed, a Mann–Whitney U test was used (MHCI). Unpaired Student's t-test ##p < 0.01, Mann–Whitney U test, *p < 0.05. Representative images of MHC class I and CD200 immunostainings, depicting the evaluation areas. DP, dermal papilla; HM, hair matrix; HS, hair shaft; ORS, outer root sheath. Scale bar, 50 μm.

Article Snippet: Antigen Primary antibody Secondary antibody Detection systemHost Manufacturer Dilution Type Manufacturer CD200 Mouse Serotec 1:250 Biotinylated goat anti-mouse Beckman Coulter TSA-FITC CK15 Mouse Chemicon 1:400 Biotinylated goat anti-mouse Beckman Coulter TSA-FITC ICAM-1 Mouse AbD Serotech 1:750 Biotinylated goat anti-mouse Beckman Coulter AEC Substrate Kit, Peroxidase (HRP) MHC class I Mouse DAKO 1:1500 Biotinylated goat anti-mouse Beckman Coulter TSA-rhodamine MHC class II Mouse DAKO 1:50 Rhodamine-labelled goat anti-mouse Jackson ImmunoResearch NGF Goat Santa Cruz 1:100 Biotinylated rabbit anti-goat Vector laboratories TSA-FITC NK1R Rabbit Abcam 1:100 Polymer-labelled HRP anti-rabbit EnVision® + System– HRP Kit, DAKO (Agilent Technologies) TRPV1/ TRPA1 Rabbit Thermo Fisher Scientific 1:50 FITC-conjugated goat anti-rabbit Jackson ImmunoResearch IF amplification: anti-fluorescein/ oregon green polyclonal (goat) antibody Alexa Fluor® 488, Invitrogen, 1:100 Abbreviations: AEC, 3-amino-9 ethylcarbazole; FITC, fluorescein isothiocyanate; TSA, Tyramide signal amplification. medulla [59–61], while most of the pigmented anagen hair bulbs remained macroscopically visible in the subcutis (Figure 2a).

Techniques: Staining, Control, Expressing, MANN-WHITNEY